Name | 3-[2-spiroadamatane]-4-methoxy-4-[3-phosphoryloxy]-phenyl-1,2-dioxetane)Dioxetane Phosphate |
Synonyms | amppd AMPPD 3-(2'-Spiroadamantan 4-Methoxy-4-(3-phosphatephenyl)spir Dia0001(3-[2-Spiroadamatane]-4-Methoxy-4-[3-.Phosphoryloxy]-Ph 4-Methoxy-4-(3-phosphatephenyl)spiro(1,2-dioxetane)-3,2'-adamantane 3-(2'-SPIROADAMANTANE)-4-METHOXY-4-(3'-PHOSPHORYLOXY)PHENYL-1,2-DIOXETANE 3-(2'-Spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane (3-[2-Spiroadamatane]-4-Methoxy-4-[3-Phosphoryloxy]-Phenyl-1,2-Dioxetane) Dioxetane 3-(4-methoxyspiro(1,2-dioxetane-3,2'-tricyclo(3.3.1.1(3,7))decan)-4-yl)phenyl phosphate 3-(4-Methoxyspiro(1,2-dioxetane-3,2'-tricyclo(3.3.1.1(3,7))decan)-4-yl)phenyl phosphate 3-[2-spiroadamatane]-4-methoxy-4-[3-phosphoryloxy]-phenyl-1,2-dioxetane)Dioxetane Phosphate 3-(4-methoxyspiro[1,2-dioxetane-3,2'-tricyclo[3.3.1.1~3,7~]decan]-4-yl)phenyl dihydrogen phosphate Phenol,3-(4-methoxyspiro[1,2-dioxetane-3,2'-tricyclo[3.3.1.13,7]decan]-4-yl)-,1-(dihydrogen phosphate) |
CAS | 122341-56-4 |
EINECS | 2017-001-1 |
InChI | InChI=1/C18H23O7P/c1-22-18(13-3-2-4-16(10-13)23-26(19,20)21)17(24-25-18)14-6-11-5-12(8-14)9-15(17)7-11/h2-4,10-12,14-15H,5-9H2,1H3,(H2,19,20,21) |
Molecular Formula | C18H23O7P |
Molar Mass | 382.34 |
Density | 1.47±0.1 g/cm3(Predicted) |
Boling Point | 548.6±60.0 °C(Predicted) |
Flash Point | 285.6°C |
Vapor Presure | 7.21E-13mmHg at 25°C |
Appearance | White fine crystal |
pKa | 1.21±0.30(Predicted) |
Storage Condition | 2-8℃ |
Refractive Index | 1.618 |
MDL | MFCD08704558 |
Introduction | 3-(2-helical adamantane) -4-methoxy-4-(3-phosphoroyl)-phenyl -1, 2-dioxirane is abbreviated as AMPPD, which is a chemiluminescent reagent. AMPPD and the familiar luminol are both redox luminescent reagents. However, AMPPD is an ultra-high sensitivity detection reagent for phosphatase, which is different from luminol, which is mainly used for blood detection. |
main properties | AMPPD is enzymatically hydrolyzed by AP under alkaline conditions to generate quite stable AMP-D anion, which has a decomposition half-life of 2-30min and emits continuous light with a wavelength of 470nm. its intensity reaches a peak at 15min and remains relatively stable within 15-60min. AMPPD is a chemiluminescent substrate of alkaline phosphatase. In a suitable buffer, with the catalytic hydrolysis of the enzyme, AMPPD decomposes into AMP-D, which emits a high intensity light signal, and its luminescence speed depends on the concentration of alkaline phosphatase. When the alkali phosphatase is coupled to the hybrid probe, the presence of the hybrid molecule can be detected by this system. |
biological activity | AMPPD is a 1, 2-dioxo-cyclohexane derivative, which is a hypersensitive alkaline phosphatase substrate. |